DentistryOnline courseEndodontics
Endodontics under the electron microscope — David Jaramillo on biofilm, histology and irrigation evidence
UTHealth Houston residency director David Jaramillo uses scanning electron and confocal microscopy images to show what actually happens inside a canal in Deep Dive Endodontics: Evidence, Histology, and Practice. Four long lessons (about 7 hours) connect caries and pulp histology, bacterial detection, biofilm in the apical third and a detailed comparison of today’s irrigation and activation systems to everyday treatment choices.
- 4lessons
- ~7 hof video
- 30+papers by the author
What you’ll be able to do
- Read SEM and confocal images of dentine, biofilm and sealer interfaces
- Relate the depth of a carious lesion to the pulp’s histological response
- Recognise and treat intraoral sinus tracts and gutta-percha extrusion
- Place, use and fully remove calcium hydroxide as an interappointment dressing
- Compare ultrasonic, PIPS, EndoVac and GentleWave irrigation on the evidence
Inside the course
Caries, pulp and diagnosis
Dens evaginatus, diagnosis of pulpal and periapical disease, CBCT detection of cracks and microscopy of early, moderate and deep lesions lead into biofilm inside tubules and canals and how to obturate lateral canals in three dimensions.
Bacteria inside and outside the root
Jaramillo explains the development of periradicular disease, uses LIVE/DEAD staining to judge bacterial viability, and shows specimens that help separate conditions with similar clinical signs.
Microbiology across treatment
Samples taken before, during and after instrumentation illustrate acute versus chronic infection, host defence against biofilm and strategies to eliminate it.
Irrigation in depth
The longest lesson (2 h 20 min) covers NaOCl, CHX and EDTA, smear layer removal, laminar versus turbulent flow, activation devices on the market and complete irrigation protocols backed by current research.
Built for
- Endodontists and residentsA research-level look at why disinfection succeeds or fails.
- General dentists doing root canalsVisual evidence to guide choice of irrigant and activation device.
Curriculum
Microscopic Evaluation of How the Dentin–Pulp Complex Reacts to Dental Caries (1 h 40 min) 11 topics
- Structural features and clinical relevance of Dens evaginatus
- Step-by-step approach to diagnosing pulpal and periapical conditions
- Microscopic evaluation of early and moderate carious lesions
- Microscopic evaluation of advanced (deep) caries
- Management considerations for caries-related pulp exposure
- Identification of crack lines using CBCT imaging
- Microscopic characteristics of asymptomatic irreversible pulpitis
- Histological assessment of biofilm within dentinal tubules
- Histological assessment of biofilm inside root canal systems
- Lateral canal obturation: common errors and prevention strategies
- Principles for achieving truly three-dimensional root canal obturation
Microscopic Detection of Intra‑ and Extraradicular Bacteria (1 h) 5 topics
- Origins and development of diseases affecting the dental pulp and surrounding periradicular tissues
- Recognition and clinical handling of intraoral sinus tracts
- Excessive extrusion of Gutta-percha: strategies to avoid it and approaches to treat complications
- Use of LIVE/DEAD™ BacLight™ staining in microscopy to evaluate bacterial viability and its practical use in endodontics
- Microscopic examination of clinical specimens with emphasis on diagnosing and differentiating relevant pathological conditions
Microscopic and microbiological evaluation of root canal systems and the pulp chamber (1 h 55 min) 9 topics
- Characteristics of infections in both their early (acute) and long-standing (chronic) forms
- Principles and use of confocal scanning-laser microscopy in endodontics
- How the body’s own defense systems respond to and combat biofilm communities
- Detailed tissue structure and morphology of the root canal system
- Microbiological evaluation of the root canal at three stages: pre-treatment, intra-treatment, and post-treatment
- Development and behavior of biofilms specifically in the apical third of the root canal
- Practical use and interpretation of images obtained through scanning electron microscopy (SEM)
- Strategies, techniques, and considerations for effective biofilm elimination
- Clinical indications for calcium hydroxide, how it is applied, and protocols for its complete removal
Emerging Approaches to Root Canal System Irrigation (2 h 20 min) 16 topics
- Core objectives and treatment outcomes in endodontic therapy
- How to locate, interpret, and manage lateral root canals
- Overview of irrigation activation systems currently on the market
- Instrument systems and techniques that optimize irrigant delivery
- Characteristics and requirements of an optimal endodontic irrigating solution
- Review of the main irrigants used today in endodontics: Sodium hypochlorite (NaOCl), Chlorhexidine (CHX), EDTA
- Strategies for effective smear layer elimination
- Application and principles of ultrasonic irrigation
- Behavior of irrigants in laminar versus turbulent flow conditions
- Principles and use of photon induced photoacoustic streaming (PIPS)
- Clinical use of the EndoUltra® cordless ultrasonic activator
- The EndoVac apical negative pressure irrigation approach
- Step-by-step root canal irrigation protocols
- Methods and considerations for dentin disinfection
- Introduction to and use of GentleWave® technology
- Evaluation of current evidence, research, and clinical studies on different irrigation systems and mechanisms
Before you buy
Is it more science than hands-on technique?
Largely yes. Most content is microscopy and literature review, although step-by-step irrigation and disinfection protocols are included.
Where has Dr Jaramillo taught?
He directs the advanced endodontic residency at UTHealth Houston and previously held full-time posts at USC and Loma Linda University.
Do I get CME credit or a certificate?
No. Clinicapedia provides the educational content only — no CME or CE credits, MOC points or certificates of completion are included with this product.
Specifications
| Format | Online video course |
|---|---|
| Instructor | David Jaramillo |
| Contents | 4 lessons |
| Duration | About 7 hours |
| Access | Lifetime, self-paced |
| Department | Dentistry |
| Delivery | Instant download · lifetime re-download |
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